Isolation and Identification of Helicobacter Pylori from RTE Foods
Dhamar Governorate in Yemen as a Case Study
Keywords:
Dhamar Governorate, Chicken sandwich, H.pylori, RTE foods, YemenAbstract
This study was designed to determine the prevalence of H. pylori in RTE foods at Dhamar Governorate from January until June 2021. Two hundred and eighty (280) samples were collected in this works; these samples included (85) chicken sandwiches, (80) Red meat sandwiches, and (115) Salad. Our result indicates that the total prevalence and isolation percentage of H. pylori from all collected samples was 37/280 (13.2%). The highest rate of pollution with H. pylori was found in Salad 17/115(14.8%), then in red meat sandwich 11/80(13.8%), while the lowest rate of occurrence was found in Chicken sandwich 9/85(10.6%). There is no significant difference between the isolation of Hp from different types of RTE foods and months. When we study the relationship between months and incidence of Hp during the research period, the results show that the highest rate of isolation of Hp from different kinds of RTE foods was found in April (18.6 %), approximately close to this rate in May (17.0 %). But the isolation rate was seen decrease whenever moved away from these three months and in both directions. There is no significant difference between the isolation of Hp from red meat and months (p>0.05).
References
Hooi J, Lai WY, Ng W K, Suen M , Underwood F E, Tanyingoh D et al. Global Prevalence of Helicobacter pylori Infection: Systematic Review and Meta-Analysis. Gastroenterology. 2017;153(2).
Azevedo NF , Guimar˜aes N, Figueiredo C , Keevil CW and Vieira MJ . A New Model for the Transmission of Helicobacter pylori:Role of Environmental Reservoirs as Gene Pools to Increase Strain Diversity. Critical Reviews in Microbiology.2007;33(3):157-169.
Mentis A, Lehours P and Mégraud F .Epidemiology and Diagnosis of Helicobacter pylori infection. Helicobacter. 2015; 20(1):1-7.
Tonkic A , Tonkic M, Lehours P , Mégraud F . Epidemiology and diagnosis of Helicobacter pylori infection. Helicobacter. 2012;17 Suppl 1:1–8.
Archampong TNA , Asmah R H , Wiredu EK , Gyasi RK , Nkrumah KN and Rajakumar K. Epidemiology of Helicobacter pylori infection in dyspeptic Ghanaian patients. Pan African Medical Journal. 2014;20(178):2-9.
Ranjbar R, Khamesipour F, Jonaidi-Jafari N, Rahimi E. Helicobacter pylori isolated from Iranian drinking water: vacA, cagA, iceA, oipA and babA2 genotype status and antimicrobial resistance properties. FEBS Open Bio. 2016; 6:433–441.
Saeidi E, Sheikhshahrokh A .VacA genotype status of Helicobacter pylori isolated from foods with animal origin. Biomed Res Int. 2016:1-6.
Al-mashhadany DA. Application of Stool Antigen Test for Monitoring Helicobacter pylori among Human in Erbil Governorate, Kurdistan Region, Iraq. Int J Pharm Pharm. 2018;10:49–3.
Khoder G, Muhammad JS , Mahmoud I, Soliman S and Burucoa C. Prevalence of Helicobacter pylori and its Associated Factors among Healthy Asymptomatic Residents in the United Arab Emirates. Pathogens (Basel, Switzerland). 2019;8(2),44 .
Cover TL, Blaser MJ. Helicobacter pylori in health and disease. Gastroenterology. 2009; 136 (6), 1863–1873. https://doi.org/10.1053/j.gastro.2009.01.073
Bae SE, Choi KD, Choe J, Kim SO, Na H K, Choi JY, et al. . The effect of eradication of Helicobacter pylori on gastric cancer prevention in healthy asymptomatic populations. Helicobacter. 2018;23(2): e12464. https://doi.org/10.1111/hel.12464
Ji J, Yang H. Using Probiotics as Supplementation for Helicobacter pylori Antibiotic Therapy. International journal of molecular sciences. 2020;21(3):11-36. https://doi.org/10.3390/ijms21031136
Sjomina O, Pavlova J, Niv Y, Leja M. Epidemiology of Helicobacter pylori infection. Helicobacter . 2018;23:e12514. https://doi.org/10.1111/HEL.12514.
Al-Mashhadany DA . Epidemiology of Helicobacter pylori among Human at Erbil Governorate/Kurdistan Region/Iraq. World J Pharm Pharm. 2020;Sci 9:435–47.
Denic M, Touati E, Reuse HDe .Pathogenesis of Helicobacter pylori infection. Helicobacter. 2020;25:e12736. https://doi.org/10.1111/HEL.12736.
Almashhadany D, Mayass SM . Prevalence of Helicobacter pylori in Human in Dhamar Governorate / Yemen. J Med Pharm Sci.2018;2:1–18.
Ma HJ , Wang JL. Quadruple therapy for eradication of Helicobacter pylori. World Journal of Gastroenterology. 2013;19(6):931-935.
EL-Guneid A, EL-Sherif AM , Murray-Lyon IM , Zureikat N , Shousha S . Effect of Chewing Qat on mucosal histology and prevalence of Helicobacter pylori in the esophagus, stomach and duodenum of Yemeni patients. Histopathology. 1991;19(5):437– 444.
Gunaid AA, Sumairi AA , Shidrawi RG , Al-Hanaki A , Al-Haimi M , Al-Absi S , et al Oesophageal and gastric carcinoma in the Republic of Yemen . British Journal of Cancer.1995;71:409–410 .
Al- Shami HZA. Prevalence of Helicobacter pylori infection among patients underwent upper gastrointestinal tract endoscopy in Sana'a major hospital .2003. M.Sc. thesis in Medical Microbiology ,Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Sana'a University, Sana'a, Yemen.
Gunaid AA , Hassan NA , Murray-Lyon L . Prevalence and risk factors for Helicobacter pylori infection among Yemeni dyspeptic patients. Saudi Medical Journal. 2003;24(5):512-517.
Al- Shamahy HA . Seroprevalence of Helicobacter pylori among children in Sana'a, Yemen. Ann Saudi Med. 2005;25(4):299-303.
Al-Makdad AM , Al-Dholaee MH , Thabet AAK, AlHaimi MA, Balfaqih OS , Al-Hadad AM . Prevalence of Helicobacter Pylori Infection in Yemeni Patients. Yemeni Journal for Medical Sciences. 2013;7:33-38.
Mayass, SMA . Prevalence of Helicobacter pylori in Human and Food and Study the Effect of Garlic Extract on Isolated Bacteria in Thamar Province. 2013; M.Sc. thesis in Microbiology, Department of Biology, Faculty of Applied Science, Thamar University, Thamar, Yemen.
Salih BA . Helicobacter pylori infection in developing countries: the burden for how long? . Saudi Journal of Gastroenterology. 2009;15(3):201-207.
Talaei R, Souod N, Momtaz H and Dabiri H. Milk of livestock as a possible transmission route of Helicobacter pylori infection. Gastroenterol Hepatol from Bed to Bench. S30. PubMed. 2015;8:26171135
Khalifa MM, Sharaf RR and Aziz RK. Helicobacter pylori: a Poor man's gut pathogen?. Gut Pathogens. 2010;2(2):2-12. http://www.gutpathogens.com/content/2/1/2.
Vale FF and Vitor JMB. Transmission pathway of Helicobacter pylori: Does food play a role in rural and urban areas? International Journal of Food Microbiology. 2010;138:1–12.
Hedlund CA. Development of new food products with components active against Helicobacter pylori – with purpose to improve gastric health in humans.2012. M.Sc. thesis in Food Science, department of food science, Faculty of Natural Resources and Agricultural Sciences, Sveriges Lantbrukssuniversitet, Swedish University of Agriculture sciences.
Abdel-Latif EF, Marouf SA and Hamza DA. Molecular And Serological Detection of Helicobacter Pylori In Cow's Milk And Its Impact On Human Health. International Journal of Biology, Pharmacy and Allied sciences (IJBPAS). 2016;5(1):60-66.
El Dairouty RK , Murad HA , El Shenawy MA, Hosny IM, Okda AY and El-Shamy SM. Helicobacter pylori and its Interrelations with other Foodborne Pathogenic Bacteria in Egyptian Meat and some Meat Products. Current Science International. 2016;5(2):139-146.
Bellack NR, Koehoorn MW, MacNab YC and Morshed MG. A Conceptual model of water's role as a reservoir in Helicobacter pylori transmission : a review of the evidence. Epidemiology and Infection Journal. 2006;134:439-449.
Doyle M . Helicobacter spp. - Food- or Waterborne Pathogens? 2012. Ph.D. thesis, University of Wisconsin- Food Research Institute, (UW FRI), Madison, Madison WI 53706, USA .
Chen SY , Liu T S , Fan XM , Dong L ,Fang GT , Tu CT , et al. Epidemiological study of Helicobacter pylori infection and its risk factors in Shanghai. Zhonghua Yi Xue Za Zhi. 2005;85(12):802–806.
Yahaghi E, Khamesipour F, Mashayekhi F, Safarpoor Dehkordi F, Sakhaei MH, Masoudimanesh M and Khameneie MK. Helicobacter pylori in vegetables and salads: genotyping and antimicrobial resistance properties. Biomed Res Int. 2014; 1-11: 757941.
Atapoor S, Safarpoor Dehkordi F and Rahimi E. Detection of Helicobacter pylori in Various Types of Vegetables and Salads. Jundishapur J Microbiol. 2014;7:e10013.
Mousavi S, Safarpoor Dehkordi F and Rahimi E. Virulence factors and antibiotic resistance of Helicobacter pylori isolated from raw milk and unpasteurized dairy products in Iran. J Venom Anim Tox inclTrop Dis. 2014;20:51.
Momtaz H, Dabiri H, Souod N and Gholami M. Study of Helicobacter pylori genotype status in cows, sheep, goats and human beings. BMC Gastroenterol. 2014;14:1–7.
Duynhoven YTHP van , Jonge R de . Transmission of Helicobacter pylori: a role for food? Bull World Health Organ. 2001;79:455–60.
Herrera AG, Helicobacter pylori and Food Products: A Public Health Problem. In: Spencer JFT, de Spencer RAL, eds. Public Health Microbiology: Methods and Protocols. Humana Press Inc, Totowa, NJ. 2004:297–301.
Al-Mashhadany D and Mayass SM. Incidence of Helicobacter pylori in food and water in Dhamar governorate / Yemen. Int J Curr Res. 2017; 9 45320–6.
Talimkhani A and Mashak Z. Prevalence and Genotyping of Helicobacter pylori Isolated From Meat, Milk and Vegetable in Iran. Jundishapur J Microbiol. 2017:10. https://doi.org/10.5812/JJM.14240.
Keenan JI, Salm N, Hampton MB and Wallace AJ . Individual and combined effects of foods on Helicobacter pylori growth. Phyther Res. 2010;24:1229–33.
Hamada M, Elbehiry A, Marzouk E, Moussa IM , Hessain AM and Alhaji JH . Helicobacter pylori in a poultry slaughterhouse: Prevalence, genotyping and antibiotic resistance pattern. Saudi J Biol Sci. 2018;25:1072–8.
Quaglia NC, Dambrosio A .Helicobacter pylori .A foodborne pathogen? World J Gastroenterol. 2018;24:3472. https://doi.org/10.1016/J.FM.2006.04.008.
Quaglia NC, Dambrosio A, Normanno G, Parisi A, Firinu A, Lorusso V and Celano GV. Survival of Helicobacter pylori in artificially contaminated ultrahigh temperature and pasteurized milk. Food Microbiol. 2007;24:296–300.
Almashhadany DA, Impact of heat treatment on the antimicrobial residues in raw goat's milk. Iraqi J Vet Sci . 2021a;35:549–3.
Almashhadany DA. Diagnosis of brucellosis in sheep and goats raw milk by fast and reliable techniques. Iraqi J Vet Sci. 2021b;35:663–8.
Lawson AJ. Helicobacter. In: Jorgensen JH, Pfaller MA, eds. Manual of Clinical Microbiology 11th ed., John Wiley & Sons, Ltd., New York, NY. 2015:1013–1027.
Rahimi E and Kheirabadi EK. Detection of Helicobacter pylori in bovine, buffalo, camel, ovine, and caprine milk in Iran. Foodborne Pathog Dis. 2012;9(5):453–6. https://doi.org/10.1089/fpd.2011.1060
Gilani A, Razavilar V, Rokni N, Rahimi E. VacA , cagA genotypes of Helicobacter pylori isolated from raw meat in Isfahan province ,Iran. VetResForum. 2017;8(1):75–80.
Kianpour F, Mehdipour, SZ, Mazroee, MA, Kazemeini HR, Rahimi E and Jafari A. Prevalence Of Helicobacter Pylori In Buffalo Milk In Iran. Trends in life sciences. An International peer – reviewed Journal. 2014;3(2):28-33.
Ghorbani F, Gheisari E, Dehkordi FS. Genotyping of vacA alleles of Helicobacter pylori strains recovered from some Iranian food items .Trop JPharmRes. 2016;15(8):1631–6. https://doi.org/10.4314/tjpr.v15i8.5.
Dore MP, Sepulveda AR, El-Zimaity H, Yamaoka Y, Osato MS, Mototsugu K, Nieddu AM, Realdi G and Graham DY. Isolation of Helicobacter pylori from sheep—implications for transmission to humans. Am J Gastroenterol. 2001;96:1396–401.
Meng X, Zhang H, Law J, Tsang R and Tsang T. Detection of Helicobacter pylori from food sources by a novel multiplex PCR assay. J. Food Saf. 2008;28:609–619.
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