Liquid Biphasic System
A Review on Modern Bioseparation Technologies
Keywords:
Aqueous biphasic system, Aqueous two-phase system, Biomolecules, Hiquid biphasic system, Purification, Separation, RecoveryAbstract
Many researchers have been interested in a substitute bioseparation technology for different biomolecules, namely the liquid biphasic system (L.B.S.). The analysis starts with a detailed discussion of the basic concept of L.B.S. and follows by a debate on the further creation of the different components for phase-forming of L.B.S. Also, various advance L.B.S. technologies will be introduced. It was addressed that is beneficial to L.B.S.'s e-function for bimolecular extraction, separation, and purification. The L.B.S. key parameters for link were obtainable and assessed. Furthermore, a guideline for future growth was described as future opportunities and challenges of L.B.S. The measures outlined in this review gives an insight into potential studies of the techniques of liquid-liquid split.
References
Khoo KS, et. al. Recent Advances in Biorefinery of Astaxanthin from Haematococcus Pluvialis. Bioresour. Technol. 2019; 288:121606.
Tham PE, et.al. Recovery of Protein from Dairy Milk Waste Product Using Alcohol-Salt Liquid Biphasic Flotation. Processes. 2019;7:875.
Azevedo AM, Rosa PA, Ferreira IF and Aires-Barros MR. Chromatography-free Recovery of biopharmaceuticals through aqueous two-phase processing. Trends Biotechnol. 2009;27:240–247.
Drexler IL and Yeh DH. Membrane applications for microalgae cultivation and harvesting: A review. Rev. Environ. Sci. Bio. 2014;13:487–504.
Grossmann, L, Ebert S, Hinrichs J and Weiss JE. ect of precipitation, lyophilization, and organic solvent extraction on the preparation of protein-rich powders from the microalgae Chlorella protothecoides. Algal. Res. 2018;29:266–276.
Sankaran R, et. al. Extraction of proteins from microalgae using the integrated method of sugaring-out assisted liquid biphasic flotation (L.B.F.) and ultrasound. Ultrason. Sonochem. 2018; 48:231–239.
Khoo KS, et. al. Extraction of natural astaxanthin from Haematococcus Pluvialis using liquid biphasic flotation system. Bioresour. Technol. 2019;290:121794.
Leong HY, et.al. Application of liquid biphasic flotation for betacyanins extraction from peel and flesh of Hylocereus polyrhizus and antioxidant activity evaluation. Sep. Purif. Technol. 2018;201:156–166.
Phong WN, Le CF, Show PL, Chang JS and Ling TC. Extractive disruption process integration using ultrasonication an aqueous two-phase system for protein recovery from Chlorella sorokiniana. Eng. Life Sci. 2017; 17:357–369.
Phong WN, et. al. Proteins recovery from wet microalgae using liquid biphasic flotation (L.B.F.). Bioresour. Technol. 2017;244:1329–1336.
Lee SY, Khoiroh I, Ling TC and Show PL. Aqueous Two-Phase Flotation for the Recovery of Biomolecules. Sep. Purif. Rev. 2016;45:81–92.
Sankaran R, et. al. Integration Process for Protein Extraction from Microalgae Using Liquid Biphasic Electric Flotation (L.B.E.F.) System. Mol. Biotechnol. 2018;60:749–761.
Koyande AK, et. al. A potential alternative to health supplementation for humans. Food Science and Human Wellness. 2019;8:16–24.
Chew KW, et. al. Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga. Bioresour. Technol. 2019;288:21519.
Van Berlo M, Luyben KCA and Van der Wielen LA. Poly (ethylene glycol)–salt aqueous two-phase systems easily recyclable volatile salts. J. Chromatogr. B Biomed. Sci. Appl. 1998;711:61–68.
Grilo AL, Raquel Aires-Barros M and Azevedo AM. Partitioning in aqueous two-phase systems: Fundamentals, applications and trends. Sep. Purif. Rev. 2016;45:68–80.
Albertsson P. Fractionation of particles and macromolecules in aqueous two-phase systems. Biochem. Pharmacol. 1961; 5:351–358.
Albertsson PA. Partitioning of Cell Particles and Macromolecules; Wiley: New York, NY, U.S.A., 1986.
Asenjo JA and Andrews BA. Aqueous two-phase systems for protein separation: Phase separation and applications. J. Chromatogr. 2012;1238:1–10.
Ooi CW, et. al. Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems. Process Biochem. 2009;44:1083–1087.
Zhao L, Peng YL, Gao JM and Cai WM. Bioprocess intensification: An aqueous two-phase process for the purification of C-phycocyanin from dry Spirulina platensis. Eur. Food Res. Technol. 2014;238:451–457.
Rosa PAJ, Ferreira IF, Azevedo AM and Aires-Barros MR. Aqueous two-phase systems: A viable platform in the manufacturing of biopharmaceuticals. J. Chromatogr. 2010:1217.
Raja, S, et. al. Aqueous two-phase systems for the Recovery of biomolecules–a review. Sci. Technol. 2011;1:7–16.
Iqbal M, et. al. Aqueous two-phase system (A.T.P.S.): An overview and advances in its applications. Biol. Proceed. Online. 2016;18:18.
Hatti-Kaul R. Aqueous Two-Phase Systems: Methods and Protocols; Springer Science & Business Media: Berlin/Heidelberg, Germany, 2000;11.
Raja S and Murty VR. Development and evaluation of environmentally benign aqueous two-phase systems to recover proteins from tannery wastewater. I.S.R.N. Chem. Eng. 2012.
Johansson HO, Feitosa E and Junior AP. Phase diagrams of the aqueous two-phase systems of poly (ethylene glycol)/sodium polyacrylate/salts. Polymers. 2011;3:587–601.
Hou D, Li Y and Cao X. Synthesis of two thermo-sensitive copolymers forming aqueous two-phase systems. Sep. Purif. Technol. 2014;122:217–224.
Tan Z, Li F, Zhao C, Teng Y and Liu Y. Chiral separation of mandelic acid enantiomers using an aqueous two-phase system based on a thermo-sensitive polymer and dextran. Sep. Purif. Technol. 2017;172:382–387.
Leong YK, et. al. Thermoseparating aqueous two-phase systems: Recent trends and mechanisms. J. Sep. Sci. 2016;39:640–647.
Show PL, et. al. Extractive fermentation for improved production and Recovery of lipase derived from Burkholderia cepacia using a term separating polymer in aqueous two-phase systems. Bioresour. Technol. 2012:116.
Ng HS, et. al. Recovery of Bacillus cereus cyclodextrin glycosyltransferase and recycling of phase components in an aqueous two-phase system using thermo-separating polymer. Sep. Purif. Technol. 2012;89:9–15.
Lin YK, et. al. Direct Recovery of cyclodextrin glycosyltransferase from Bacillus cereus using aqueous two-phase flotation. J. Biosci. Bioeng. 2015;120:684–689.
Jong WYL, Show PL, Ling TC and Tan YS. Recovery of lignin peroxidase from submerged liquid fermentation of Amauroderma rugosum (Blume & T. Nees) Torrent using polyethene glycol/salt aqueous two-phase system. J. Biosci. Bioeng. 2017;24:91–98.
Lin YK, et. al. Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems. Sep. Purif. Technol. 2013;120:362–366.
Chia SR, et. al. Sustainable approach in phlorotannin recovery from macroalgae. J. Biosci. Bioeng. 2018;126:220–225.
Smiglak M, Metlen A and Rogers RD. The Second Evolution of Ionic Liquids: From Solvents and Separations to Advanced Materials Energetic Examples from the Ionic Liquid Cookbook. A. Chem. Res. 2007;40:1182–1192.
Lee SY, et. al. Evaluating self-bu using ionic liquids for biotechnological applications. A.C.S. Sustain. Chem. Eng. 2015;3:3420–3428.
Zhang S, et. al. Physical properties of ionic liquids: Database and evaluation. J. Phys. Chem. Ref. Data 2006;35:1475–1517.
Lee SY, et. al. Recent advances in protein extraction using ionic liquid-based aqueous two-phase systems. Sep. Purif. Rev., 2017;46:291–304.
Ostadjoo S, Berton P, Shamshina JL and Rogers RD. Scaling-up ionic liquid-based technologies: How much do we care about their toxicity? Prima facie information on 1-ethyl-3-methylimidazolium acetate. Toxicol. Sci. 2017;161:249–265.
Sun, N, et. al. Complete dissolution and partial delignification of wood in the ionic liquid 1-ethyl-3-methylimidazolium acetate. Green Chem. 2009;11:646–655.
Swatloski RP, Spear SK, Holbrey JD and Rogers RD. Dissolution of cellulose with ionic liquids. J. Am. Chem. Soc. 2002;124:4974–4975.
Gutowski KE, et. al. Controlling the aqueous miscibility of ionic liquids: Aqueous biphasic systems of water-miscible ionic liquids and water-structuring salts for recycling, metathesis, and separations. J. Am. Chem. Soc. 2003;125:6632–6633.
Du Z, Yu YL and Wang JH. Extraction of proteins from biological fluids using an ionic liquid/aqueous two-phase system. Chem. A Eur. J., 2007;13:2130–2137.
Ng HS, et. al. Recovery of Bacillus cereus cyclodextrin glycosyltransferase using the ionic liquid-based aqueous two-phase system. Sep. Purif. Technol. 2014;138:28–33.
Chang YK, et. al. Isolation of C-phycocyanin from Spirulina platensis microalga using the Ionic liquid-based aqueous two-phase system. Bioresour. Technol. 2018;270:320–327.
Garcia ES, et. al. Fractionation of proteins and carbohydrates from crude microalgae extracts using an ionic liquid based-aqueous two-phase system. Sep. Purif. Technol. 2018;204:56–65.
Abbott AP. Deep eutectic solvents formed between choline chloride and carboxylic acids: versatile alternatives to ionic liquids. J. Am. Chem. Soc. 2004;126:9142–9147.
Shishov A, et. al. Application of deep eutectic solvents in analytical chemistry. A review. Microchem. J., 2017;135:33–38.
Paiva A, et. al. Natural deep eutectic solvents–solvents for the 21st century. A.C.S. Sustain. Chem. Eng. 2014;2:1063–1071.
Dai Y, et. al. Natural deep eutectic solvents new potential media for green technology. Anal. Chim. Acta, 2013;766:61–68.
Pang, J, et. al. Green aqueous biphasic systems containing deep eutectic solvents and sodium salts for the extraction of protein. R.S.C. Adv. 2017;7:49361–49367.
Zeng Q, et. al. Deep eutectic solvents as novel extraction media for protein partitioning. Analyst, 2014;139:2565–2573.
Zhang X, Teng G and Zhang J. Deep eutectic solvents aqueous two-phase system based ultrasonically assisted extraction of ursolic acid (U.A.) from Cynomorium solarium Rupr. Chem. Eng. Commun. 2019;206:419–431.
Weschayanwiwat P, Kunanupap O and Scamehorn JF. Benzene removal from wastewater using aqueous surfactant two-phase extraction with cationic and anionic surfactant mixtures. Chemosphere, 2008;72:1043–1048.
Selber K, et. al. Large-scale separation and production of engineered proteins, designed for facilitated Recovery in detergent-based aqueous two-phase extraction systems. Process Biochem. 2004;39:889–896.
Amid M, Manap M, Hussin M and Mustafa S. A novel aqueous two-phase system composed of surfactant and xylitol for the purification of lipase from pumpkin (Cucurbita moschata) seeds and recycling of phase components. Molecules 2015;20:11184–11201.
Sankaran R, et. al. Green technology of liquid biphasic flotation for enzyme recovery utilizing recycling surfactant and sorbitol. Clean Technol. Environ. Policy 2018;20:2001–2012.
Sebba F. Ion Flotation; Elsevier: Amsterdam, The Netherlands. 1962; 30.
Sankaran R, et. al. Integration process of fermentation and liquid biphasic flotation for lipase separation from Burkholderia cepacia. Bioresour. Technol. 2018;250:306–316.
Wu X, Joyce EM and Mason TJ. The e ects of ultrasound on cyanobacteria. Harmful Algae, 2011;10:738–743.
Wang, M, et. al. Disruption of microalgal cells using high-frequency focused ultrasound. Bioresour. Technol. 2014;153:315–321.
Gerde JA, et. al. Evaluation of microalgae cell disruption by ultrasonic treatment. Bioresour. Technol. 2012;125:175–181.
Chemat F, et. al. Ultrasonics Sonochemistry Ultrasound-assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason. Sonochem. 2017;34:540–560.
Pakhale SV, Vetal MD and Rathod VK. Separation of bromelain by aqueous two-phase flotation. Sep. Sci. Technol. 2013;48:984–989.
Rahim AHA, Khoo KS, Yunus NM and Hamzah WSW. Ether-Functionalized Ionic Liquids as Solvent for Gigantochloa Scortechini Dissolution. Proceedings of A.I.P. Conference Proceedings, Cesme-Izmir, Turkey, 26–30 May 2019:020025.
Dong B, et. al. Ultrasound-assisted aqueous two-phase extraction of phenylethanoid glycosides from Cistanche deserticola Y.C. Ma stems. J. Sep. Sci. 2015;38:1194–1203.
King CJ, et. al. Ects of nanosecond pulsed electric field exposure on Arabidopsis thaliana. IEEE Trans. Dielectr. Electr. Insul. 2009;16:1322–1328.
Kotnik T, et. al. Electroporation-based applications in biotechnology. Trends Biotechnol. 2015;33:480–488.
Luengo E, Condón-Abanto S, Álvarez I and Raso JE. Ect of pulsed electric field treatments on permeabilization and extraction of pigments from Chlorella Vulgaris. J. Member. Biol. 2014;247:1269–1277.
Leong HY, et. al. Integration process for betacyanins extraction from peel and flesh of Hylocereus polyrhizus using liquid biphasic electric flotation system and antioxidant activity evaluation. Sep. Purif. Technol. 2019;209:193–201.
Lam GP, et. al. Mild and selective protein release of cell wall deficient microalgae with the pulsed electric field. A.C.S. Sustain. Chem. Eng. 2017;5:6046–6053.
Phong WN, Show PL, Chow YH and Ling TC. Recovery of biotechnological products using aqueous two-phase systems. J. Biosci. Bioeng. 2018;126:273–281.
Santos SB, et. al. Selective partition of caffeine from coffee bean and guaraná seed extracts using alcohol–salt aqueous two-phase systems. Sep. Sci. Technol. 2016;51:2008–2019.
Huang L, et. al. Simultaneous recovery of glycyrrhizic acid and liquidity from Chinese liquorice root (Glycyrrhiza uralensis Fisch) by the aqueous two-phase system and evaluation biological activities of extracts. Sep. Sci. Technol. 2018;53:1342–1350.
Wang Y, Liu Y, Han J and Hu S. Application of water-miscible alcohol-based aqueous two-phase systems for extraction of dyes. Sep. Sci. Technol. 2011;46:1283–1288.
Wingfield P. Protein precipitation using ammonium sulfate. Curr. Protoc. Protein Sci. 1998;13, A. 3F. 1–A. 3F. 8.
Lu, Y.; Yu, M.; Tan, Z. and Yan, Y. Phase equilibria and salt e ect on the aqueous two-phase polyoxyethylene system cetyl ether and sulfate salt at three temperatures. J. Chem. Eng. Data 2016, 61, 2135–2143.
Yang L, et. al. Purification of plant-esterase in PEG1000/NaH2PO4 aqueous two-phase system by a two-step extraction. Process Biochem. 2010;45:1664–1671.
Goja AM, Yang H, Cui M and Li C. Aqueous two-phase extraction advances for bioseparation. J. Bioprocess. Biotechnol. 2013;4:1–8.
Andrews B, Schmidt A and Asenjo J. Correlation for the partition behaviour of proteins in aqueous two-phase systems: E ect of surface hydrophobicity and charge. Biotechnol. Bioeng. 2005;90:380–390.
Olivera-Nappa A, Lagomarsino G, Andrews BA and Asenjo JA. Ect of electrostatic energy on the partitioning of proteins in aqueous two-phase systems. J. Chromatogr. B 2004;807:81–86.
Chakraborty A and Sen K. Impact of pH and temperature on phase diagrams of different aqueous biphasic systems. J. Chromatogr. 2016;1433:41–55.
Chow YH, et. al. Characterization of bovine serum albumin partitioning behaviours in polymer-salt aqueous two-phase systems. J. Biosci. Bioeng. 2015;120:85–90.
Khoo KS, et. al. Liquid Biphasic System: A Recent Bioseparation Technology. Processes. 2020;8:149.
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